Evercode™ Combinatorial Barcoding

Scalable single cell without the instrument

Evercode™ split-pool combinatorial barcoding enables you to scale up your single cell projects to millions of cells or nuclei. Learn more about how the technology uniquely labels cells without ever needing to isolate individual cells.

Exponentially scalable

Evercode's combinatorial barcoding enables you to dramatically scale up the cells and samples per experiment.

No instrument required

If you have a centrifuge, thermal cycler, and some pipettes, you’re ready to go.

Unmatched data quality

Better detect lowly expressed genes and avoid ambient RNA common in droplet-based single cell sequencing.

Fixation of cells/nuclei

Fix and store samples as they come in for up to 6 months and then run together later on your schedule. Ideal for time-courses and cross-site collaborations.

Combinatorial barcoding with Evercode™

It all starts with Evercode™ split-pool combinatorial barcoding, our proprietary technology that labels molecules with cell-specific combinations of barcodes.

Cells are first fixed and permeabilized, turning them into their own reaction vessels, removing the need to capture individual cells in droplets or microwells. The split-pool barcoding process then labels cells with an exponentially large number of barcode combinations making it possible to easily scale beyond other technologies. Up to 1 million cells, collected on different days, can be processed in parallel. Discover your lab’s full potential.

Reverse Transcription

SplitSamples are distributed into wells and the first, sample-specific barcodes are applied to fixed cells or nuclei with an in-cell reverse transcription (RT) reaction.

PoolCells from each well are pooled together.

Ligation

SplitCells or nuclei are distributed across a plate and an in-cell ligation appends the second barcode.

PoolCells from each well are pooled together.

Ligation

SplitThe third barcode is applied with another in-cell ligation after the cells or nuclei are split across a plate.

PoolCells from each well are pooled together.

Lysis + PCR

SplitThe pooled cells are divided across several sublibraries. The cells are lysed and the fourth, sublibrary-specific barcode is applied by PCR.

Library Prep

SplitThe library preparation appends adapters ready for loading on any  next-generation sequencer.

Travel through time with fixation

Fix cells or nuclei to lock in the biology until your experiment is ready. In timecourse studies, avoid uncertainty and remove batch effects by running samples collected on different days together.

Fix and store your samples for up to 6 months

Fixation locks in the biology to provide workflow flexibility. We checked a freshly prepared sample against the same sample stored for 6 months to prove stability of the fixation and reproducibility of the assay (Evercode™ WT v1 results shown).

Unmatched sensitivity at any scale

Run more samples. Use less sequencing. Delve deeper into the biology with gene and transcript detection that outperforms droplet-based methods. Evercode™ combinatorial barcoding technology works inside individual cells in a highly parallel fashion, resulting in unmatched data quality regardless of experimental size.

Better gene detection than droplet-based approaches

Evercode WT v2 detected an average of 84% more genes than the Chromium Next GEM Single Cell 3’ Kit v3.1 at the common read depth target of 20,000 reads/cell in mouse brain.

Interactive insights are part of the package

Every kit comes with our data analysis package, which transforms sequencing output into understandable results. Assess data quality, identify sample differences, interrogate genes of interest, and seamlessly upload data into popular tools like Seurat or Scanpy. An interactive web report allows anyone to easily browse the data and share results with collaborators.

Dive deeper into your data

Whole Transcriptome

Immune Profiling

Additional Capabilities

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Technology

The end-to-end solution

Products

Whole Transcriptome

Immune Profiling

Additional Capabilities

Evercode™ Low Input Fixation

Resources

Trailmaker

Company

Recent News

Parse Biosciences Welcomes Admera Health as Newest Member of Certified Serv...

Parse Biosciences Launches Evercode Mouse BCR to Support Antibody Engineeri...

Scalable single cell without the instrument

Exponentially scalable

No instrument required

Unmatched data quality

Fixation of cells/nuclei

Combinatorial barcoding with Evercode™

Reverse Transcription

Ligation

Ligation

Lysis + PCR

Library Prep

Travel through time with fixation

Fix and store your samples for up to 6 months

Unmatched sensitivity at any scale

Better gene detection than droplet-based approaches

Interactive insights are part of the package