EVERCODE™ T-CELL RECEPTOR (TCR) MEGA SINGLE CELL PAIRED TCR + WHOLE TRANSCRIPTOME

Scalable immune profiling, 1 million cells at a time

1M

Cells

96

Samples

When dealing with a system as complex as the full repertoire of immunity, more is better. Achieve unpresecented resolution as you study TCR clonotype diversity and T-cell states. Evercode™ TCR Mega offers scale alongside a host of other benefits, with the added information provided by WT profiles.

Explore example datasets

1M

Cells

96

Samples

Evercode™ TCR Mini

Up to 10K cells, 1-12 samples

Evercode™ TCR

10K-100K cells, 1-48 samples

Evercode™ TCR Mega

100K-1M cells, 1-96 samples

Evercode™ TCR Mini

Up to 10K cells, 1-12 samples

Evercode™ TCR

10K-100K cells, 1-48 samples

Evercode™ TCR Mega

100K-1M cells, 1-96 samples

Single cell TCR profiling + whole transcriptome—without the instrument

Reveal the complexity of the immune repertoire with sensitive detection of TCRs. Measure transcriptome-wide gene expression (including key T-cell subtype markers) together with paired alpha and beta sequences in the same cells.

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Up to 1 million cells in a single experiment

Scale matters. Uncovering the vast diversity in the immune repertoire requires large cell experiments. Identify more TCR clonotypes than ever before with Evercode.

MAP CLONOTYPES TO T CELL SUBTYPE CLUSTERS

Gene expression on nearly 1 million T cells isolated from PBMCs. In addition to capturing their repertoire, cells can be classified into  T cell subtypes.

EVERCODE™ TECHNOLOGY MAKES IT ALL POSSIBLE

A simpler way to scale your single cell immune profiling

Evercode™ split-pool combinatorial barcoding is a simple, instrument-free workflow that converts the cell or nucleus into an individual reaction compartment. This easily adopted approach brings unprecedented sensitivity, scalability, and flexibility to any lab.

Exponentially scalable

Evercode's combinatorial barcoding enables you to dramatically scale up the cells and samples per experiment.

No instrument required

If you have a centrifuge, thermal cycler, and some pipettes, you’re ready to go.

Unmatched data quality

Evercode provides sensitive detection of TCRs along with whole transcriptome profiles.

Works with fixed cells and nuclei

Fix and store samples as they come in for up to 6 months and then run together later on your schedule. Ideal for time-courses and cross-site collaborations.

Learn more about the technology

High TCR detection in activated and primary T cells

Isolated T-cells from peripheral blood mononuclear cells (PBMCs) were either directly profiled (Primary) or placed in culture for 3 days and activated with CD3/CD28 beads and IL-2 (Activated).

Comprehensive immune repertoire detection

The Evercode TCR kit captures the largest single cell immune repertoire dataset from a single experiment.

I compared technologies and I'm using Parse TCR for our next experiments

Christoph T. Ellebrecht

University of Pennsylvania

The Evercode™ TCR workflow

The Evercode™ TCR solution provides the reagents, software, and accessibility to pursue difficult research questions.

Fixation

Lock in gene expression immediately after sample collection with a rapid fixation protocol. After fixation, samples can be stored for up to 6 months or proceed directly to barcoding.

Barcoding & Library Prep

Append barcodes to each transcript by progressing cells through a streamlined split-pool combinatorial barcoding process, which produces sequencing-ready libraries.

Sequencing

The resulting libraries are sequenced by NGS.

Data Analysis

Our computational pipeline generates an interactive report for rapid insights. All output data files, including gene-cell count matrix, integrate seamlessly with existing open source tools such as Seurat or Scanpy.

TCR Mega: Resources and support

Get the Brochure

Read more about the technology and dive deeper into the technical details.

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Information and insight for the entire range of Parse Biosciences Products.

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Reach out for a quote or for help planning your next experiment.

Technology

The end-to-end solution

Products

Whole Transcriptome

Immune Profiling

Additional Capabilities

Evercode™ Low Input Fixation

Resources

Trailmaker

Company

Recent News

Parse Biosciences Welcomes Admera Health as Newest Member of Certified Serv...

Parse Biosciences Launches Evercode Mouse BCR to Support Antibody Engineeri...

Technology

The end-to-end solution

Products

Whole Transcriptome

Immune Profiling

Additional Capabilities

Evercode™ Low Input Fixation

Resources

Trailmaker

Company

Recent News

Parse Biosciences Welcomes Admera Health as Newest Member of Certified Serv...

Parse Biosciences Launches Evercode Mouse BCR to Support Antibody Engineeri...

Scalable immune profiling, 1 million cells at a time

1M

96

1M

96

Evercode™ TCR Mini

Evercode™ TCR

Evercode™ TCR Mega

Evercode™ TCR Mini

Evercode™ TCR

Evercode™ TCR Mega

Single cell TCR profiling + whole transcriptome—without the instrument

Up to 1 million cells in a single experiment

MAP CLONOTYPES TO T CELL SUBTYPE CLUSTERS

A simpler way to scale your single cell immune profiling

Exponentially scalable

No instrument required

Unmatched data quality

Works with fixed cells and nuclei

High TCR detection in activated and primary T cells

Comprehensive immune repertoire detection

I compared technologies and I'm using Parse TCR for our next experiments

Christoph T. Ellebrecht

The Evercode™ TCR workflow

Fixation

Barcoding & Library Prep

Sequencing

Data Analysis

TCR Mega: Resources and support

Get the Brochure

Support

We're your partners in single cell