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Discover scalable, instrument-free single cell sequencing technology from Parse Bioscience

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Providing researchers single cell sequencing with unprecedented scale and ease

About Parse

EVERCODE™ TCR
SINGLE CELL PAIRED TCR + WHOLE TRANSCRIPTOME

More insights with more scalable single cell TCR profiling

Up to 1M

Cells

Up to 96

Samples

Explore a wide variety applications across the adaptive immune system through Evercode™ TCR. Without needing to purchase proprietary hardware, your lab can delve into the immune response, detecting paired alpha-beta chains to characterize the immune repertoire complexity.

Explore datasets

Up to 1M

Cells

Up to 96

Samples

Evercode™ TCR Mini

Up to 10K cells, 1-12 samples

Evercode™ TCR

10K-100K cells, 1-48 samples

Evercode™ TCR Mega

100K-1M cells, 1-96 samples

Evercode™ TCR Mini

Up to 10K cells, 1-12 samples

Evercode™ TCR

10K-100K cells, 1-48 samples

Evercode™ TCR Mega

100K-1M cells, 1-96 samples

Capture paired TCRs and gene expression from the same cell at unprecedented scale

Reveal the complexity of the immune repertoire with sensitive detection of TCRs. Measure transcriptome-wide gene expression (including key T-cell subtype markers) together with paired alpha and beta sequences in the same cells.

1M cells in 1 experiment

Capture the diverse immune repertoire with the power of 1 million T cells. The Evercode TCR Mega kit enables the study of TCR clonotype diversity and T cell states at an unprecedented resolution.

EVERCODE™ TECHNOLOGY MAKES IT ALL POSSIBLE

A simpler way to scale your single cell immune profiling

Evercode™ split-pool combinatorial barcoding is a simple, instrument-free workflow that converts the cell or nucleus into an individual reaction compartment. This easily adopted approach brings unprecedented sensitivity, scalability, and flexibility to any lab.

Exponentially scalable

Evercode's combinatorial barcoding enables you to dramatically scale up the cells and samples per experiment.

No instrument required

If you have a centrifuge, thermal cycler, and some pipettes, you’re ready to go.

Unmatched data quality

Evercode provides sensitive detection of TCRs along with whole transcriptome profiles.

Works with fixed cells and nuclei

Fix and store samples as they come in for up to 6 months and then run together later on your schedule. Ideal for time-courses and cross-site collaborations.

Sensitive clonotype detection

Understanding the immune repertoire diversity necessitates sensitive detection of TCRs along with whole transcriptome profiles.

In an experiment profiling viral or autoantigen stimulated T cells, Evercode TCR captured paired alpha and beta chains in 85-94% of cells.

Measuring clonotype diversity over time

Over half a million unique beta chain clonotypes were identified across 7 timepoints in mouse lifespan, showing a clear decrease in clonotype diversity with age.

Simplify TCR profiling with fixation

Parse makes it easy to batch samples with fixation. With Evercode TCR, fix and store samples as they come in and then run the samples together later on your schedule.

I compared technologies and I'm using Parse TCR for our next experiments


Christoph T. Ellebrecht

University of Pennsylvania

The Evercode™ TCR workflow

The Evercode™ TCR solution provides the reagents, software, and accessibility to pursue difficult research questions.

1
Fixation

Lock in gene expression immediately after sample collection with a rapid fixation protocol. After fixation, samples can be stored for up to 6 months or proceed directly to barcoding.

2
Barcoding & Library Prep

Append barcodes to each transcript by progressing cells through a streamlined split-pool combinatorial barcoding process, which produces sequencing-ready libraries.

3
Sequencing

The resulting libraries are sequenced by NGS.

4
Data Analysis

Our computational pipeline generates an interactive report for rapid insights. All output data files, including gene-cell count matrix, integrate seamlessly with existing open source tools such as Seurat or Scanpy.

Resources

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