EVERCODE™ BCR
SINGLE CELL PAIRED B CELL RECEPTOR + WHOLE TRANSCRIPTOME

Single cell BCR profiling at scale

Up to 1M

Cells

Up to 96

Samples

Explore a wide variety of adaptive immunity applications with Evercode™ BCR - the only instrument-free, fixed sample BCR profiling solution that detects full-length, natively paired heavy and light chain sequences to comprehensively characterize the BCR repertoire.

Explore datasets

Up to 1M

Cells

Up to 96

Samples

Evercode™ BCR Mini

Up to 10K cells, 1-12 samples

Evercode™ BCR

10K-100K cells, 1-48 samples

Evercode™ BCR Mega

100K-1M cells, 1-96 samples

Evercode™ BCR Mini

Up to 10K cells, 1-12 samples

Evercode™ BCR

10K-100K cells, 1-48 samples

Evercode™ BCR Mega

100K-1M cells, 1-96 samples

Capture full length BCR sequences and gene expression together at unprecedented scale

Reveal the full complexity of the immune repertoire with sensitive detection of BCRs. Measure transcriptome-wide gene expression together with full-length paired heavy and light sequences and isotypes in the same cells.

1M B cells in a single experiment

Scale up your experiments to capture the full complexity of the immune repertoire. Here, Evercode BCR simultaneously profiled over a million isolated human B cells from 24 donors including individuals with Type-1 Diabetes, Multiple Sclerosis, Rheumatoid Arthritis, Crohn's, and Celiac disease.

ONE KIT WITH ENDLESS APPLICATIONS

A simpler way to scale your single cell immune profiling

From high-throughput BCR sequencing and clonotype tracking to antibody discovery and immune response analysis, Evercode BCR is your partner in a wide range of applications. Powered by Evercode split-pool combinatorial barcoding technology, it delivers the sensitivity, scalability, and flexibility needed for comprehensive BCR analysis.

BCR diversity meets cell identity

Connect BCR repertoire and cell type identity for deeper insights into cellular context and functional relevance, even in rare populations.

Unrivaled paired-chain resolution

Detect full-length, natively-paired immunoglobulin heavy and light chain sequences for a complete view of the BCR repertoire.

Cross-species compatible

Broad model compatibility supports human, mouse, and humanized transgenic mouse research to bridge basic and preclinical applications.

Scalable
and flexible

The only instrument-free, fixed-sample BCR profiling assay streamlines processing with the flexibility for any size lab or collaboration.

Insights into isotype switching

As the only all-in-one solution for humanized transgenic mice, Evercode BCR delivers unprecedented resolution of human-mouse chimeric BCRs in Atlas™ mice revealed 35% class-switched clonotypes of plasmablasts following tetanus toxoid immunization.

Paired heavy and light chain detection at scale

Resolution of paired full-length heavy and light chain BCRs is essential for classifying B cell clonality. In an experiment profiling T1D and control donors, Evercode BCR captured both heavy and light chains with 87-89% BCR pairing efficiency.

1 Experiment. >900,000 Unique clonotypes.

The Evercode BCR kit generated the most comprehensive immune repertoire detection from a single experiment to date. Over 900,000 unique paired clonotypes were identified across the 24 donors with the vast majority being classified as unique clonotypes.

The Evercode™ BCR workflow

The Evercode™ BCR solution provides the reagents, software, and accessibility to pursue difficult research questions.

Fixation

Lock in gene expression immediately after sample collection with a rapid fixation protocol. After fixation, samples can be stored for up to 6 months or proceed directly to barcoding.

Barcoding & Library Prep

Append barcodes to each transcript by progressing cells through a streamlined split-pool combinatorial barcoding process, which produces sequencing-ready libraries.

Sequencing

The resulting libraries are sequenced by NGS.

Data Analysis

Our computational pipeline generates an interactive report for rapid insights. All output data files, including gene-cell count matrix, integrate seamlessly with existing open source tools such as Seurat or Scanpy.

Resources

Get the Brochure

Read more about the technology and dive deeper into the technical details.

Download the brochure

Support

More information and literature across the entire range of Parse Biosciences products.

Support Suite

We're your partners in single cell

Reach out for a quote or for help planning your next experiment.

Technology

The end-to-end solution

Products

Whole Transcriptome

Immune Profiling

Additional Capabilities

Evercode™ WT Penta

Resources

Trailmaker

Company

Recent News

Parse Biosciences Launches 5 Million Cell Evercode WT Penta Kit for Single ...

Parse Biosciences Invalidates All 10x Genomics’ Patent Claims Asserted Agai...

Technology

The end-to-end solution

Products

Whole Transcriptome

Immune Profiling

Additional Capabilities

Evercode™ WT Penta

Resources

Trailmaker

Company

Recent News

Parse Biosciences Launches 5 Million Cell Evercode WT Penta Kit for Single ...

Parse Biosciences Invalidates All 10x Genomics’ Patent Claims Asserted Agai...

Single cell BCR profiling at scale

Up to 1M

Up to 96

Up to 1M

Up to 96

Evercode™ BCR Mini

Evercode™ BCR

Evercode™ BCR Mega

Evercode™ BCR Mini

Evercode™ BCR

Evercode™ BCR Mega

Capture full length BCR sequences and gene expression together at unprecedented scale

1M B cells in a single experiment

A simpler way to scale your single cell immune profiling

BCR diversity meets cell identity

Unrivaled paired-chain resolution

Cross-species compatible

Scalableand flexible

Insights into isotype switching

Paired heavy and light chain detection at scale

1 Experiment. >900,000 Unique clonotypes.

The Evercode™ BCR workflow

Fixation

Barcoding & Library Prep

Sequencing

Data Analysis

Resources

Get the Brochure

Support

We're your partners in single cell

Scalable
and flexible