Comparison of Evercode™ WT v3 and Chromium™ GEM-X Single Cell 3’ Kit v4 in Mouse Brain Nuclei

Comparison Highlights

• Head-to-head sensitivity comparison shows a superior gene detection with Evercode WT v3.
• Cell type proportions are equivalently represented.
• Evaluation of differentially expressed genes reveals that Evercode detects twice as many differentially expressed genes.

Experimental Design

Two embryonic mouse brain samples were collected, sagittally dissected into 2 halves, and flash frozen by a third-party tissue vendor. Half of each brain was shipped to a 10x Genomics certified service provider to isolate nuclei and create sequencing libraries. The other halves of each brain were processed by Parse Biosciences for nuclei isolation, fixation, and library preparation. Sequencing libraries from each technology were sequenced by a third-party. The sequencing data were analyzed by each manufacturer’s data analysis pipeline.