Evercode™ WT v3 and Chromium™ Next GEM Single Cell 3’ Kit v3.1 in Mouse Lymph Node Nuclei

Key Tech Note Takeaways

• Head-to-head sensitivity comparison shows increased gene detection with Evercode WT v3
• Integrated results show consistent gene expression and cell type proportions
• More differentially expressed genes detected in every cell type with Evercode WT v3

Experimental Design

Mouse lymph node tissue was dissociated into a single nuclei solution with a Singulator™ 100/200 (S2 Genomics). The samples were strained, centrifuged, and resuspended in S2 Genomics Nuclei Storage Buffer and RNase Inhibitor. The sample was split, and half of the sample was prepared with the 10x Genomics Next GEM Single Cell 3’ Kit v3.1. The remaining half was fixed with Evercode Cell Fixation v3 and shipped for further processing with Evercode WT v3. The sequencing data were processed with each manufacturer’s respective analysis pipeline.

Explore the Data