Technology

Discover scalable, instrument-free single cell sequencing technology from Parse Bioscience

Technology Overview


Resources

Explore our collection of resources to learn more about technology and its applications from leading researchers

Resources Overview

Company

Providing researchers single cell sequencing with unprecedented scale and ease

About Parse

Evercode™ WT PENTA V3
SINGLE CELL WHOLE TRANSCRIPTOME

Unparalleled accessibility and scale for single cell research

5M

Cells

384

Samples

Scale single cell experiments without compromise. Evercode™ WT Penta empowers research with limitless possibilities by enabling the unbiased discovery of novel gene expression profiles and previously uncharacterized cell types.

Register for the launch webinar

5M

Cells

384

Samples

EVERCODE™ TECHNOLOGY MAKES IT ALL POSSIBLE

Unbounded single cell discovery, at your fingertips

Evercode™ split-pool combinatorial barcoding is a simple, instrument-free solution to single cell sequencing. This easily adopted approach brings unprecedented sensitivity, scalability, and flexibility to any lab.

Exponentially scalable

Evercode's combinatorial barcoding enables you to dramatically scale up the cells and samples
per experiment.

No instrument required

If you have a centrifuge, thermal cycler, and some pipettes, you’re ready to go.

Unmatched data quality

Better detect lowly expressed genes and avoid ambient RNA common in droplet-based single cell sequencing.

Works with fixed cells and nuclei

Fix and store samples as they come in for up to 6 months and then run together later on your schedule. Ideal for time-courses and cross-site collaborations.

Learn more about the technology

Consistently low doublet rates across all throughputs

Multiplets introduce noise and compromise the integrity of single cell data. With Evercode technology, where the cell itself is the reaction vessel, doublet rates remain consistently low across all our kits—ensuring cleaner, more reliable data. This means higher confidence in rare cell population detection, more precise clustering, and better overall resolution of cellular heterogeneity.

Minimize batch effects. Ease logistics.

With Evercode’s fixation technology, researchers can preserve biological states at the moment of collection, regardless of species, locking in gene expression profiles. This flexibility means samples don’t need to be processed immediately, allowing labs to coordinate with core facilities, or scale up experiments without logistical constraints. By fixing now and processing later, researchers can more feasibly expand their studies—exploring more samples, conditions, or time points—without sacrificing data quality.

We detected rare cell types with Evercode’s higher sensitivity


Yi Xie

Duke-NUS Medical School

Enrico Petretto

Duke-NUS Medical School

Jacques Behmoaras

Duke-NUS Medical School

The Parse Biosciences workflow

The Evercode™ Whole Transcriptome solution provides the reagents, software, and support to pursue difficult research questions from bench to insight.

1

Fixation

Lock in gene expression immediately after sample collection with a rapid fixation protocol. After fixation, samples can be stored for up to 6 months or proceed directly to barcoding.

2

Barcoding & Library Prep

Append barcodes to each transcript by progressing cells through a streamlined split-pool combinatorial barcoding process, which produces sequencing-ready libraries.

3

Sequencing

The resulting libraries are sequenced by NGS.

4

Data Analysis

Our Trailmaker platform provides an end-to-end data analysis experience, guiding you from FASTQ files to publication-ready figures, while maintaining the flexibility of multiple data entry and exit points. Our computational pipeline continues to also be available.

Support

More information and literature across the entire range of Parse Biosciences products.

We're your partners in single cell

Reach out for a quote or for help planning your next experiment.