Technology

Discover scalable, instrument-free single cell sequencing technology from Parse Bioscience

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Providing researchers single cell sequencing with unprecedented scale and ease

About Parse

EVERCODE™ T-CELL RECEPTOR (TCR) MINI SINGLE CELL PAIRED TCR + WHOLE TRANSCRIPTOME

Get started with single cell TCR profiling, without the instrument

10K

Cells

12

Samples

The Evercode™ TCR Mini is perfect to start your single cell immune repertoire sequencing project. Generate single cell TCR and gene expression data without needing to purchase an expensive droplet-based instrument.

Explore example datasets

10K

Cells

12

Samples

Single cell TCR profiling + whole transcriptome—without the instrument

Reveal the complexity of the immune repertoire with sensitive detection of TCRs. Measure transcriptome-wide gene expression (including key T-cell subtype markers) together with paired alpha and beta sequences in the same cells.

EVERCODE™ TECHNOLOGY MAKES IT ALL POSSIBLE

A simpler way to scale your single cell immune profiling

Evercode™ split-pool combinatorial barcoding is a simple, instrument-free workflow that converts the cell or nucleus into an individual reaction compartment. This easily adopted approach brings unprecedented sensitivity, scalability, and flexibility to any lab.

Exponentially scalable

Evercode's combinatorial barcoding enables you to dramatically scale up the cells and samples per experiment.

No instrument required

If you have a centrifuge, thermal cycler, and some pipettes, you’re ready to go.

Unmatched data quality

Evercode provides sensitive detection of TCRs along with whole transcriptome profiles.

Works with fixed cells and nuclei

Fix and store samples as they come in for up to 6 months and then run together later on your schedule. Ideal for time-courses and cross-site collaborations.

High TCR detection in activated and primary T cells

Isolated T cells from peripheral blood mononuclear cells (PBMCs) were either directly profiled (Primary) or placed in culture for 3 days and activated with CD3/CD28 beads and IL-2 (Activated).

I compared technologies, and I'm using Parse TCR for our next experiments


Christophe T Ellebrecht

University of Pennsylvania

The Evercode™ TCR workflow

The Evercode™ TCR solution provides the reagents, software, and accessibility to pursue difficult research questions.

1
Fixation

Lock in gene expression immediately after sample collection with a rapid fixation protocol. After fixation, samples can be stored for up to 6 months or proceed directly to barcoding.

2
Barcoding & Library Prep

Append barcodes to each transcript by progressing cells through a streamlined split-pool combinatorial barcoding process, which produces sequencing-ready libraries.

3
Sequencing

The resulting libraries are sequenced by NGS.

4
Data Analysis

Our computational pipeline generates an interactive report for rapid insights. All output data files, including gene-cell count matrix, integrate seamlessly with existing open source tools such as Seurat or Scanpy.

TCR Mini: Resources and support

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